PRK5 VECTOR MAP PDF

Plasmid pRK5-HA-Ubiquitin-WT from Dr. Ted Dawson’s lab contains the insert Ubiquitin C The wild-type ubiquitin sequence in this plasmid is: MQIFVKTLTG. 18 May DESCRIPTION. The pRK-5 vector contains a powerful cytomegalovirus promoter and is designed for high level expression of cloned genes in. Visit ChemicalBook To find more PRK-5 MAMMALIAN EXPRESSION VECTOR() information like chemical properties,Structure,melting point,boiling point,density.

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All IRES selection vectors contain a partially disabled IRES which reduces the efficiency of translation initiation for the selection marker relative to that of the cloned gene and allows preferential selection of cells expressing high levels of your protein of interest. The antibody recognizes an epitope located within residues — Co-immunoprecipitation of myc-tagged and HA-tagged proteins in mammalian cells Strong constitutive expression Confirm yeast two-hybrid interactions in mammalian cells Strong, clean bands prk5 vector map Western blot.

The myc-tagged protein of interest can be detected by either western analysis or fluorescence microscopy using an anti-myc antibody. However this promoter is not present in the packaged viral RNA genome. IRES bicistronic expression vectors permit your gene of interest and a selection marker to be translated from a single mRNA.

Create an account to get started. Used to select for cell lines that have been transfected with vectors containing the puromycin-N-acetyl-transferase pac resistance gene. Log in to enjoy additional benefits Why sign up for an account?

Table summarizing results from the varying viral vectors produced in this study.

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Selectable mammalian expression vectors

Jonathan A Luong, Email: This product is intended for research purposes only. We have also developed this lentiviral vector with a multiple cloning site so that any transgene can be easily cloned into this prk5 vector map. The transfection efficiency using the leading competitor product, on the other hand, is no higher than background.

Twenty-four hours after transfection of hZACwe observed a reduced number of cells, compared with mock transfected cells data prk5 vector map shown.

Back Hygromycin B Hygromycin B. This vector has a promoter from cytomegalovirus CMV IE promoter and polyA signal from herpes simplex virus thymidine kinase.

PRK-5 MAMMALIAN EXPRESSION VECTOR |

This vector has a promoter from the human EF-1 alpha promoter and polyA signal from herpes simplex virus thymidine kinase. Pr5 Components Image Data Back Puromycin supplied in mg and mg formats Puromycin supplied in mg and mg formats. Therefore if neuronal specific gene expression is desired, then transgenes within VSV-G pseudotyped viruses must be controlled by neuronal specific promoters.

Jonathan E Ploski, Email: The antibiotic resistance marker, either puromycin or neomycin, is expressed separately from an SV40 promoter. Back Our customers have indicated via survey that they have successfully used Xfect Transfection Reagent to transfect the cell lines listed here Our customers have indicated via survey that they have successfully used Xfect Transfection Reagent to transfect the cell lines listed here.

Lentiviruses were produced using the following lentiviral vector plasmids: Evctor prk5 vector map encoding human veftor c- src 21 was provided by D. Capturem Trypsin Columns may be used to completely digest protein samples in less than pprk5 minute with digestion efficiencies protein coverage comparable to or better than those obtained using in-solution trypsin digestion.

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Transfections can be carried out entirely in the presence of serum. Recommended dose for selection and maintenance in mammalian cell lines. This vector contains the internal ribosome entry site IRES of the encephalomyocarditis virus ECMVwhich permits both the gene of vectr and the puromycin selection marker to be translated from a single mRNA.

The vectof Vector is designed for the efficient production of stable cell lines expressing recombinant proteins in mammalian cells. Alternatively, lentiviruses derived from human immunodeficiency virus type rpk5 HIV-1are single prk5 vector map RNA viruses that are prk5 vector map pseudotyped with the vesicular stomatitis virus G-protein VSV-G and the VSV-G capsid provides the virus the ability to transduce many cell types.

The pBApo series of vectors are mammalian expression vectors for constitutive expression of your gene of interest from either a CMV or EF1-alpha promoter. Back Different plasmid purification technologies yield endotoxin levels appropriate for individual applications Different plasmid purification technologies yield endotoxin levels appropriate for individual applications.

Received Dec prk5 vector map Accepted Feb 2. We use cookies to improve your browsing experience and provide meaningful content.

Susceptibility of cells vecctor hygromycin B varies with cell type, proliferative activity, and other parameters; therefore, we suggest performing a full dose-response curve for each cell system used.